【摘要】 建立番泻叶滴丸中番泻苷B的含量测定方法.采用高效液相色谱法测定含量;ODS柱,流动相A为乙腈,B为醋酸-醋酸钠缓冲溶液(pH值为5.0)(1∶10),加入3.77 g四庚基溴化氨,A与B的比例为14∶86(V∶V),检测波长为340 nm,流速为1.0 mL/min.番泻苷B的线性范围为0.4~1.4μg(r=0.9998),回收率(n=6)为98.33%(RSD=0.6%).本方法简便准确,重现性好.更多还原

【Abstract】 To establish a method for determining sennoside B in dropping pill of Folium Sannae.The determination is performed by HPLC using ODS column,mobile phase is a mixture of diluted pH value is 5.0 acetic acid-sodium acetate buffer solution(1∶10) and acetonitrile(86∶14,V∶V) containing 7.7 mmol/L tetra-n-heptylammonium bromide.Detection wavelength is 340 nm and a flow rate of 1.0 mL/min.The linearity range is 0.4～1.4 μg(r=0.999 8).The average recovery is 98.33%(n=6),RSD=0.6%.The method is simple,accurate and highly reproducible.更多还原