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紫外分光光度法测定长白楤木根、茎、叶中多糖含量
Determination of the Polysaccharide in Roots,Stalks and Leaves of Aralia Continentalis Kitagwa by Spectrophotometry
【摘要】 采用紫外分光光度法于490nm波长测定长白楤木根、茎、叶中所含多糖的含量。结果其回归方程为:A=0.0062C-0.0118,r=0.9992;平均加样回收率为99.19%(RSD=1.72%),测定三年生植株含糖量9.09%。表明该方法简便、准确,可用于长白楤木的质量控制。
【Abstract】 To establish a method for the determination of the polysaccharide in Roots, Stalks and Leaves of Aralia continentalis Kitagwa. The content of polysaccharide was determined by spectrophotometric at 490nm wavelength.Results indicated the curve was A=0.0062C-0.0118,r=0.9992, the average recovery was 99.19% and RSD was 1.72%(n=5).The content of polysaccharide in three- year-old plants was 9.09%. This method was easy and accurate,and can be used for quality control of Aralia continentalis Kitagwa.
【关键词】 长白楤木;
紫外分光光度法;
多糖;
【Key words】 aralia continentalis kitagwa; spectmphotometric method; polysaccharide;
【Key words】 aralia continentalis kitagwa; spectmphotometric method; polysaccharide;
【基金】 黑龙江省农垦总局项目(HNKXIV-06-06-04)
- 【文献出处】 黑龙江八一农垦大学学报 ,Journal of Heilongjiang Bayi Agricultural University , 编辑部邮箱 ,2009年03期
- 【分类号】S647
- 【被引频次】11
- 【下载频次】265