【摘要】 0hpf斑马鱼胚胎经50μg/L五氯酚(PCP)暴露染毒8 h,提取其总RNA,与Affymetrix公司的斑马鱼基因组芯片杂交.发现与对照组相比,染毒样本中1 149个基因的表达显著增强(Signal log ratio>1),501个基因的表达显著减弱(Signal log ratio<-1).利用Chipinfo、Genmapp等生物信息学软件对差异表达基因进行了基因本体论功能分类和发育调控通路分析,结果显示,显著差异表达的基因涉及抗氧化、信号传导、翻译调节、转录调节等多个分子功能.五氯酚暴露后斑马鱼胚胎发育重要的调控通路BMP信号、ERK通路、FGF信号、Nodal信号的调控基因smad2、smad5、bmp4、bmp7f、lh、n-ras的表达发生了显著变化,Signal logratio分别为4.6、2.1、1.6、1.0、1.3、1.0.这些结果为进一步阐明五氯酚的发育毒性作用机制提供了标志物.更多还原

【Abstract】 0hpf zebrafish embryos were exposed to 50(μg/L) pentachlorophenol(PCP) for 8h in vitro.Total RNA sample was extracted then and hybridized with Affymetrix Zebrafish Genome Array representing approximately(14?900) transcripts.A total of(1?149) transcripts was significantly up-regulated while 501 transcripts were down-regulated.Bioinformatic tools were used for further analysis.The result indicated that genes with significant expression changes were related to molecular functions including antioxidant activity,signal transducer activity,translation regulator activity,transcription regulator activity,et al.Genes regulated by BMP signals,FGF signals,and Nodal signals including smad2,smad5,bmp4,bmp7,flh,n-ras may involve in the developmental toxicity of PCP,with Signal log ratios of 4.6,2.1,1.6,1.0,1,1.3,1.0,respectively.This investigation may provide new biomarkers to further study of the developmental toxicity of PCP.更多还原