【摘要】 从某地盐场及三聚氯氰工厂采集的水样和泥样中分离得到嗜盐菌10株,细胞呈杆状、球状和弧状.根据嗜盐菌在废水中的生长状况,选取4株长势良好的嗜盐菌组成复合嗜盐菌,用于三聚氯氰废水动态模拟试验.对这4株嗜盐菌进行了形态观察、革兰氏染色及各项生理生化指标分析,并与Sherlock微生物鉴定系统(Microbial Identification System,MIS)的结果进行比对,得出这4株嗜盐菌分别属于盐单胞菌属、假单胞菌属和芽孢杆菌属.通过改变培养条件(如温度,pH和盐度),得出复合嗜盐菌的最佳生长条件:在37℃下其最适pH为7~9,ρ(盐)为70~170 g/L,属于中度嗜盐菌.在复合嗜盐菌最佳生长条件下,动态模拟装置经过72 h的生物降解,系统中氨氮、氰化物和TOC的去除率分别为90.47%,97.62%和97.06%.更多还原

【Abstract】 Ten strains of halophilic bacteria with rod,globular and vibrio shapes were isolated from wastewater and sludge of saltern and cyanuric chloride factories and researched.Four strains with better growth states were selected and mixed to form a bacterial complex,which was used for dynamic simulated tests on cyanuric chloride containing wastewater.These four halophilic bacteria were studied by microscopic observation,Gram staining,and biochemistry analysis,and compared with the results from the Sherlock microbial identification system.The results showed that the four stains belonged to Halomonas,Pseudomonas,and Bacillus.The best growth conditions of these complex bacteria were determined as: temperature(37 ℃),pH 7-9,and salt mass concentration ranking from 70 to 170(g/L),indicating that these bacteria were moderately halophilic.After 72 hours’ biodegradation,the removal rates of ammonia nitrogen,cyanide and TOC were 90.47%,97.62% and 97.06%,respectively,under the best growth conditions in the dynamic simulated test.更多还原