【摘要】 分别用Folin-酚改进法、考马斯亮兰法和紫外分光光度法等3种测试方法测试了上海市某小区生活污水中的蛋白质含量,对这3种测试方法进行了比较,并采用氨基酸仪分析了生活污水中蛋白质水解后的氨基酸化学组成。试验结果表明,Folin-酚改进法测试蛋白质浓度的准确性与精确性优于考马斯亮兰法和紫外分光光度法,可用于测定生活污水中蛋白质的浓度;氨基酸仪法测得该生活污水中含有17种氨基酸,其中最多的为谷氨酸(3.6 mg/L),含量最低的是脯氨酸(0.8 mg/L);所测生活污水中蛋白质COD占总COD的18.9%。更多还原

【Abstract】 Proteins in domestic wastewater were measured by three methods(modified Folin-phenol method,Bradford method and spectrophotometer method).The chemical composition of proteins in domestic wastewater were also investigated with amino acids analyzer.The results showed that the Folin-phenol method was a more sensitive and accurate method than Bradford method and spectrophotometer method in determining proteins in domestic sewage.The wastewater was analyzed by amino acids analyzer after being hydrolyzed to amino acids.The amino acids analyzer method gave us the information about the amino acids that constitute proteins in wastewater.There are 17 kinds of amino acids determined in the wastewater.Among the amino acids,the concentration of glutamic acid is 3.6 mg/L,which is the highest of all the amino acids;the lowest is praline acids(0.8 mg/L).The results also indicated that the COD of proteins is about 18.9 percents of the total COD in the domestic wastewater.更多还原