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Klebsiella pneumoniae发酵菊芋生产2,3-丁二醇的初步研究

Preliminary Study on Fermentative Production of 2,3-Butanediol from Jerusalem artichoke Tubers by Klebsiella pneumoniae

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【作者】 孙丽慧王旭东戴建英修志龙

【Author】 SUN Li-hui, WANG Xu-dong, DAI Jian-ying, XIU Zhi-long(Dept.Biosci.Biotechnol., Sch.Environ.Biolog.Sci.Technol., Dalian Univ.Technol., Dalian, Liaoning 116024, China)

【机构】 大连理工大学环境与生命学院生物科学与工程系

【摘要】 对Klebsiella pneumoniae发酵菊芋块茎生产2,3-丁二醇进行了初步研究,通过摇瓶实验考察了不同碳源及培养基中微量元素对发酵的影响.结果表明,菊芋是良好的发酵2,3-丁二醇的底物,以其为底物时产物浓度和生产强度比葡萄糖发酵提高了42%以上,培养基中不添加微量元素对菊芋发酵基本没有影响,因而可简化培养基成分以降低生产成本.在发酵罐批式流加实验中,发酵56h菊芋发酵的产物浓度和生产强度分别为81.47g/L和1.45g/(L-h),与葡萄糖发酵结果相当.

【Abstract】 2,3-Butanediol production from Jerusalem artichoke tubers by Klebsiella pneumoniae was investigated.Shaking flask cultures were performed in order to study the effects of different carbon sources and trace elements on the fermentation of 2,3-BD.The results showed that Jerusalem artichoke was an excellent carbon source for the production of 2,3-BD, which provided more than 42% higher concentration and productivity of target products, compared with glucose used as carbon source.Moreover, there was no distinct difference between added and none trace elements in the medium.The fed-batch fermentation results showed that the concentration and productivity of target products from Jerusalem artichoke reached 81.47 g/L and 1.45 g/(L-h), respectively after 56 h fermentation, which were close to that from glucose.

【关键词】 2,3-丁二醇菊芋发酵
【Key words】 2,3-butanediolJerusalem artichokefermentation
  • 【文献出处】 过程工程学报 ,The Chinese Journal of Process Engineering , 编辑部邮箱 ,2009年01期
  • 【分类号】TQ923
  • 【被引频次】27
  • 【下载频次】346
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