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落葵多糖含量测定方法的研究

Study on Methods for Determination of Polysaccharide Content from Basella

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【作者】 林爱琴郭善慧陈少昆

【Author】 Lin Aiqin Guo Shanhui* Chen Shaokun(Fuqing Branch of Fujian Normal University,Fujian Fuqing 350300;*Shishi office of Fujian Exit—Entry Inspection and Quarantine Bureau,Fujian Shishi 362700)

【机构】 福建师范大学福清分校福建出入境检验检疫局石狮办事处

【摘要】 比较了糖含量两种测定方法,苯酚-硫酸法和蒽酮-硫酸法,以葡萄糖为对照品,并测定落葵中多糖含量。结果显示,苯酚-硫酸法测定糖含量的精密度、稳定性均优于蒽酮-硫酸法,并用此法测得落葵多糖的含量为5.35%,加样平均回收率100.70%,RSD 1.15%。

【Abstract】 Two methods for Determination of Polysaccharide content from Basella was studied.Phenol-sulfuric acid method and anthrone-sulfuric acid method were used to compare the stability and accuracy of the absorption curse after chromogenic reaction.The results showed that stability and accuracy of the phenol-sulfuric acid method was better.Basella polysaccharide content of 5.35%,the average yield=100.70%,the recovery RSD=1.15% were determined by this method.

【基金】 福建省教育厅科研资助项目(JB08251)
  • 【文献出处】 化工时刊 ,Chemical Industry Times , 编辑部邮箱 ,2009年08期
  • 【分类号】R151
  • 【被引频次】9
  • 【下载频次】203
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