【摘要】 利用DNA测序技术,在荷斯坦奶牛胎儿中找到X染色体基因GLRA2 3’端的杂合位点(C/T),设计错配引物在该位点对胎儿及其父本、母本基因组进行PCR扩增,建立起GLRA2基因的错配PCR技术,该技术可以快速在群体基因组中检测这一杂合位点.更多还原

【Abstract】 Using DNA sequencing,a heterozygous locus(C/T) was identified at the 3’ region of GLRA2 gene,a X-linked housekeeping gene in cow.To further validate this SNP(single-nucleotide polymorphism) locus,the technique of mismatch PCR was established and amplified to examine the samples of embryo,paternal and maternal genomic DNA.The result suggests that this technique can also be applied to verify the same locus in the cow population.更多还原