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喜树内生真菌的分离及产10-羟基喜树碱菌的鉴定
Isolation and identification of a 10-hydroxycamptothecin-producing endophytic fungus from Camptotheca acuminate Decne
【摘要】 从喜树(Camptotheca acuminate Decne)树皮中分离、纯化得到6株内生真菌,经摇瓶培养后,采用TLC法与HPLC法对其菌丝体提取物进行分析,结果表明其中1株内生真菌(XK002)产10-羟基喜树碱(10-hydroxycam ptothecin,HCPT),将此菌株命名为XK002,其10-羟基喜树碱产量为410μg/L.对菌株XK002菌落、菌丝体和孢子的有无进行了研究,表明菌株XK002属于无孢菌群(Mycelia sterlia).
【Abstract】 Six endophytic fungi isolated from Camptotheca acuminate Decne were cultured in taper flask under shaking. The organic extracts of mycelium were analyzed with thin layer chromatography (TLC) and high performance liquid chromatography (HPLC). The results showed one of the strains produced the 10-hydroxycamptothecin (HCPT) with output of 410 μg/L and was named XK002. Morphological observation indicates that XK002 belongs to Mycelia sterlia.
【关键词】 喜树内生真菌；
分离；
鉴定；
10-羟基喜树碱；
【Key words】 endophytic fungi of Camptotheca acuminate Decne； isolation； identification； 10-hydroxycamptothecin；
【Key words】 endophytic fungi of Camptotheca acuminate Decne； isolation； identification； 10-hydroxycamptothecin；
- 【文献出处】 华东师范大学学报(自然科学版) ,Journal of East China Normal University(Natural Science) , 编辑部邮箱 ,2009年05期
- 【分类号】S182
- 【被引频次】33
- 【下载频次】239