【摘要】 目的:观察肺炎支原体(MP)诱导A549细胞产生肿瘤坏死因子-α(TNF-α)和白细胞介素-8(IL-8)的相关性,为MP肺炎的临床诊治提供依据。方法:体外培养A549细胞和MP,用感染复数为1:20的MP加入到A549细胞中共同孵育,采用双抗体夹心ELISA法检测不同刺激时间的MP作用A549细胞后分泌TNF-α和IL-8的含量。结果:MP能显著诱导A549细胞分泌TNF-α和IL-8(均P<0.01),经相关回归分析,其产生的水平与刺激时间具有依赖性(r=0.821,0.911,P<0.01),且二者之间具有量的正相关(r=0.942,P<0.01)。结论:MP诱导A549细胞产生的炎性细胞因子TNF-α和IL-8具有量的正相关性。更多还原

【Abstract】 Objective: To investigate the relation of cytokines tumor necrosis factor-α(TNF-α) and interleukin-8(IL-8) secretions in A549 cells which were induced by Mycoplasma pneumoniae(MP).Methods: A549 cells and MP were cultured in vitro.MP of multiplicity of infection(MOI)1:20 were co-cultured with A549 cells in vitro.Enzyme-linked immunosorbent assay(ELISA) was used to detect the contents of TNF-α and IL-8 in different time.Results: MP could significantly induce TNF-α and IL-8 secretions in A549 cells(P<0.01,respectively).With the correlation and regression analysis,the level of TNF-α or IL-8 was respectively depended on stimulation time(r=0.821,P<0.01;r=0.911,P<0.01).And there was a positive correlation between the content of TNF-α and IL-8(r=0.942,P<0.01).Conclusion: There is a positive correlation between the cytokine TNF-α and IL-8 secretions induced by MPs in A549 cells.更多还原