【摘要】 目的:探讨小檗碱预防颈动脉粥样硬化形成的作用机制。方法:将24只雄性新西兰大白兔随机分成3组:正常组、颈动脉粥样硬化组(模型组)、小檗碱预防组(小檗碱组)。正常组给予正常饮食,模型组行高脂饲料喂养加颈动脉内膜空气干燥术建立颈动脉粥样硬化模型,小檗碱组在给予模型组相同处理的同时灌服小檗碱。术后4周麻醉处死,取颈动脉组织行HE染色、NF-κBP65免疫组织化学染色、RT-PCR法测NF-κBP65mRNA表达水平。结果:模型组血管病变以泡沫细胞为主,有动脉粥样斑块形成;小檗碱组血管主要显示内膜明显增厚,有少量泡沫细胞;小檗碱组NF-κBP65阳性细胞数密度高于正常组(P<0.05),明显低于模型组(P<0.05);RT-PCR半定量分析发现,小檗碱组NF-κBP65/β-actin扩增带吸光度值比值高于正常组(P<0.05),而明显低于模型组(P<0.05)。结论:小檗碱可能通过抑制NF-κB活性,降低组织中NF-κB含量,预防颈动脉粥样硬化形成。更多还原

【Abstract】 Objective: To explore the mechanism of prevention of Berberine on the carotid artery atherosclerosis.Methods: Twenty-four New Zealand white rabbits were divided into normal group,carotid artery atherosclerosis(model group),and Berberine prevention group(Berberine group).The model group and Berberine group were fed with high cholesterol diet and the carotid artery atherosclerosis models were established by air-dry method,then the Berberine group were treated with Berberine respectively.After four weeks,all rabbits were executed with anaesthesia.All the carotid arteries were performed HE staining,immunohistochemistry staining and RT-PCR.Results: Model group were characterized with foam cells which formed atheromatous plaque,while berberine group showed thickening in intima with only few foam cells underneath.The number density of the positive cells and NF-κB p65 mRNA /β-actin in berberine group was decreased compared with that in model group respectively(both P<0.05).Conclusion: Berberine can deplete activity of nuclear factor kappa B,decrease the level of nuclear factor kappa B,so as to prevent the development of carotid artery atherosclerosis.更多还原