【摘要】 目的:比较正常胃黏膜、残胃炎、胃癌、残胃癌中hMLH1、hMSH2、c-Myc及PTEN蛋白表达情况,探讨上述蛋白在残胃癌发生、发展中的作用。方法:用免疫组化SP法检测hMLH1、hMSH2、c-Myc、PTEN蛋白在20例正常胃黏膜、20例残胃炎、38例胃癌、18例残胃癌组织中hMLH1、hMSH2、c-Myc、PTEN蛋白的表达情况。结果:在残胃癌中hMLH1、hMSH2蛋白的阳性率分别为50.0%,44.4%,分别低于正常胃黏膜组织100.0%,100.0%(P<0.01)。胃癌中hMLH1、hMSH2的表达阳性率为68.4%,63.2%,与残胃癌相比无显著差异(P>0.05)。c-Myc蛋白在残胃炎黏膜组织的表达阳性率(45.0%)要高于正常胃黏膜(0.0%)(P<0.05),而残胃癌中的表达阳性率(61.1%)与胃癌中的表达(55.3%)差异无显著性(P>0.05)。PTEN蛋白在残胃癌(44.4%)和胃癌(47.4%)中的表达均下调,两组之间差异无统计学意义(P>0.05)。结论:残胃癌的发生可能与错配修复系统失活有关,癌基因c-myc激活和抑癌基因PTEN的失活也参与了残胃癌的发生、发展。更多还原

【Abstract】 Objective: To compare the expression of hMLH1,hMSH2,c-Myc and PTEN in normal gastric mucosa,gastric remnant mucosa,gastric remnant cancer(GRC) and gastric cancer(GC),and to investigate their roles in carcinogenesis and development of gastric remnant cancer.Methods: The SP immunohistochemical method was applied to detect the expression rate of hMLH1,hMSH2,c-myc and PTEN gene in normal gastric mucosa,gastric remnant mucosa,gastric remnant cancer and gastric cancer.Results: The expression rate of hMLH1(50.0%) and hMSH2(44.4%) was significantly decreased in GRC,compared with that in normal gastric mucosa(100.0% and 100.0% respectively)(P<0.01),while was not different from that in GC(68.4% and 63.2%,respectively).The positive expression of c-Myc in gastric remnant mucosa(45.0%) was higher than in normal gastric mucosa(0.0%)(P<0.05).There was no difference in positive expression rate of c-Myc protein between GRC(61.1%) and GC(55.3%).The positive expression rate of PTEN was observed and was downregulated in GRC(44.4%) and GC(47.4%),but no significant differences were observed between the two groups.Conclusion: GRC might be associated with the inactivation of mismatch repair gene.Increased expression of c-Myc protein and reduced expression of PTEN protein might be important in the tumorigenesis and progression of GRC.更多还原