【摘要】 通过研究胎衣不下奶牛胎盘组织中孕酮和雌激素的含量变化,探讨了奶牛发生胎衣不下的内分泌学机制。将20头奶牛分为健康对照组和胎衣不下组,分别采集健康奶牛和胎衣不下奶牛的胎儿胎盘,制备胎盘组织匀浆液,用酶联免疫吸附法(ELISA)检测其中的孕酮和雌激素的含量。结果发现,胎衣不下组奶牛胎盘组织中的孕酮含量高于健康对照组奶牛,差异极显著(P<0.01);而雌激素含量低于健康对照组,差异极显著(P<0.01)。表明胎盘组织中孕酮和雌激素的含量与奶牛胎衣不下的发生密切相关。更多还原

【Abstract】 To study the change regulation of placenta progesterone and estrogen in retained placenta of cows,twenty cows were divided into two groups. The foetal placentas of cows were collected. The contents of progesterone and estrogen in foetal placenta supernatant were measured by enzyme linked immuno sorbent assay(ELISA) . The results showed that the level of progesterone of placenta retention group was significantly higher than that of control group(P<0.01) ;however,the level of estrogen of retained placenta group was significantly lower than that of the control group(P<0.01) . The results demonstrated that placenta retention of cows was associated with the level of progesterone and estrogen in placenta.更多还原