【摘要】 本试验对构建的辣椒育性恢复相关基因的消减cDNA文库进行质量分析。通过对文库构建过程中的各个环节,包括总RNA的提取,mRNA的分离纯化,RasⅠ酶切,接头连接,消减效率检测以及菌落PCR等,进行分析和评估,证明利用抑制消减杂交技术(SSH)所构建的消减文库是准确可靠的,富集了大量与辣椒育性恢复相关的基因,为进一步精确筛选、克隆、分析辣椒育性恢复相关基因奠定了基础。更多还原

【Abstract】 To evaluate the subtracted library of fertility restoration-related genes in pepper,a quality analysis was conducted,including total RNA extraction,mRNA purification,RasⅠdigestion,adaptor ligation efficiency analysis,PCR analysis of subtraction efficiency,and colony PCR.The result was that the subtracted library constructed using suppression subtractive hybridization(SSH)was accurate and reliable,and had the large number of pepper fertility restoration-related genes.更多还原