【摘要】 从福建、浙江、湖南、宁夏、安徽、山西、河南、河北、北京等省市采集并收集到16个黄瓜花叶病毒(CMV)样品,用亚组特异性抗血清以ELISA方法对这些样品进行了亚组鉴定。同时,选取亚组Ⅰ、Ⅱ病毒部分分离物进行了症状、致病性和体外稳定性比较研究。结果表明,亚组Ⅱ病毒分离物的症状和致病性受温度、湿度和光照强度等的调节,在不同环境条件下呈现复杂的寄主适应性变化,总的症状比较温和。我国收集的CMV样品以亚组Ⅰ为主,检出率占样品总数的75%。但由两亚组病毒生物学特性比较研究结果表明,亚组Ⅱ病毒在采样和检测中易于被忽略或丢失,据此,探讨了CMV亚组Ⅱ存在的生态意义及其样品检出率与田间实际发生率之间可能存在的较大差异。更多还原

【Abstract】 16 CMV samples from Fujian,Zhejiang,Hunan,Ningxia,Anhui,Shanxi,Henan,Hebei and Binjing were collected and identified by DAS-ELISA with subgroup specific Antisera.The symptoms,pathygenicities,and in vivo stabilities of part of the isolates were compared in Nicotiana tabacum.The results showed that the majority of the CMV samples belong to the subgroup Ⅰ virus,consisting of 75% of the samples.The symptoms and pathygenicities of subgroup Ⅱ virus were generally mild and flexible,and adjusted by temperature,humidity and light intensity,suggesting its complex flexibility to the host plant.These results suggested that the subgroup Ⅱ virus was prone to be neglected or discarded in sample collecting or experimenting.The differences between detection frequency and actual existence,also the ecological significance of subgroup Ⅱ virus were discussed.更多还原