【摘要】 为了探讨热激蛋白在低温锻炼诱导的耐热性形成过程中的保护功能,进一步分析葡萄幼苗对温度逆境产生交叉适应性的细胞学机制,试验以葡萄(Vitis vinifera L. cv. Jingxiu)幼苗为试材,采用胶体金免疫电镜定位技术,定位观察了小分子热激蛋白17.6(sHSP17.6)在葡萄叶片中的亚细胞分布情况,结果表明,低温锻炼预处理可增强其在高温胁迫条件下的表达水平,细胞核和叶绿体中代表sHSP17.6的免疫金颗粒密度比相应的对照细胞明显增多,尤其是胁迫处理3 h时。这一结果为sHSP17.6参与低温锻炼诱导的耐热性提供了更直观的细胞学证据。更多还原

【Abstract】 In order to investigate the protecting role of small heat shock protein 17.6(sHSP17.6)in the heat tolerance induced by cold acclimation(CA),and then to explore the mechanism of cross adaptation to different temperature stresses in grape plants.Leaves from two-year-old young grape (Vitis vinifera L.cv.Jingxiu)plants were used as experimental materials.The subcellular localizations of sHSP17.6 within mesophyll cells were observed using immuno-gold labeling electron microscopic technique.The results showed that CA pretreatment could enhance the expression level of sHSP17.6 within heat stress.Compared to non-acclimated plants,the density of sHSP17.6 gold particles was obviously increased in the nucleus and chloroplast of the CA leaves,especially 3 h after heat stress.Which offered the cytological evidence of sHSP17.6 was contributed to the heat tolerance induced by CA pretreatment in grape plants.更多还原