【摘要】 利用pc12细胞的抗抑郁损伤模型,研究了槲皮素的抗抑郁作用。槲皮素、氯丙咪嗪与0.1 mmol.L-1皮质酮共孵pc12细胞48 h,用MTT比色法判断细胞损伤程度。结果表明,高浓度皮质酮处理pc12细胞后,细胞存活率比正常细胞降低;当给予槲皮素或氯丙咪嗪后,细胞存活率升高,表明细胞损伤减少或接近正常。与经典抗抑郁剂氯丙咪嗪的作用效果一样,槲皮素对皮质酮损伤的pc12细胞有明显的保护作用,有抗抑郁作用。更多还原

【Abstract】 The antidepressant effect of quercetin on the cultured pc12 cells lesioned by corticosterone was studied.Cultured pc12 cells were treated with 0.1 mmol·L-1 corticosterone(Cort) for 48 h at the presence of quercetin or clomipamraminemine hydrochloride(Clom).The cellular viability was determined by using MTT assay.After incubation with 0.1 mmol·L-1 Cort,the cellular viability was decreased;While at the presence of quercetin or clomipamraminemine hydrochloride,the cellular viability was all improved,indicating that treatment with quercetin or Clom could protect pc12 cells lesioned by Cort.Just the same as clomipamraminemine hydrochloride,quercetin also could antagonize the lesion induced by Cort in pc12 cells.The results extended evidence that quercetin had the effect of antidepressant.更多还原