【摘要】 目的探讨多参数流式细胞术对淋巴结良恶性病变鉴别的价值。方法病理诊断恶性淋巴瘤(肿瘤组)20例,无病变淋巴结(非肿瘤组)20例,将40例淋巴结石蜡标本制成单细胞悬液,流式细胞术检测FSC和SSC坐标系中较一致细胞群是否存在,并用PI染色对特征性细胞群的DNA倍体进行检测。结果1)特征性细胞群检测:肿瘤组与非肿瘤组出现率分别为85.0%和25.0%,两组阳性率差异有统计学意义(P<0.01)。2)DNA倍体检测:肿瘤组出现特征性细胞群17例中非整倍体DNA 13例;非肿瘤组出现特征性细胞群5例中均为整倍体DNA,两组之间比较差异有统计学意义(P<0.05)。联合使用两种方法灵敏度为(76.5±10.3)%,特异度为100%。结论多参数流式细胞术有助于鉴别诊断淋巴结良恶性病变,可作为常规病理诊断的辅助检测。更多还原

【Abstract】 Objective To evaluate multi-parameter flow cytometry measurement on differentiating malignant lymphoma from benign ones.Methods Twenty cases of pathological diagnosed lymphoma were selected with 20 benign cases as control.The paraffin embedded tissues were made into single cell suspension,and then detected by Flow cytometry(FCM) with display in the coordination system by FSC and SSC to see whether or not the characteristic cell group(CCG) exists.If so,another experiment on DNA ploidy based on DNA index was performed by PI staining.Results 1) CCG test: the occurrence rates of CCG in groups of malignant lymphoma and control were 85.0% and 25.0% respectively with statistical significance(P<0.01).2) DNA ploidy detection: All samples with CCG were tested the ploidy by FCM.Thirteen of 17 lymphomas and 0 of 5 controls were found heteroploid with statistical significance(P<0.05).Combined manipulate these two methods reached sensitivity of 76.5±10.3% and specificity of 100%.Conclusion It was helpful using multi-parameter FCM to differentiate malignant lymphoma from benign mimics and may be considered as a useful assist for common pathological processes.更多还原