【摘要】 目的观察大鼠早期脑缺血再灌注损伤后细胞凋亡情况及尼膜同对其影响。方法将24只SD大鼠随机分成3组:假手术组,尼膜同组,对照组,每组各8只。采用线栓法建立大鼠局灶性脑缺血再灌注模型。假手术组不作缺血处理,给予正常剂量麻醉剂;对照组缺血30 min后腹腔注射生理盐水2 ml;尼膜同组缺血30 min后腹腔注射生理盐水稀释的尼膜同注射液,剂量1 mg/kg。24 h后进行神经功能缺陷评分,原位末端标记法(TUNEL)测定细胞凋亡阳性数。结果假手术组偶见凋亡细胞,对照组见大量凋亡细胞,尼膜同组凋亡细胞数明显减少(P<0.05)。结论尼膜同可抑制大鼠神经细胞凋亡,对大鼠早期脑缺血再灌注损伤有一定的保护作用。更多还原

【Abstract】 Objective To investigate the effects of nimotop on apoptosis of neuronal cell in early period of focal brain ischemia reperfusion injury in rats.Methods 24 SD rats were divided randomly into three groups: sham operative group,control group and nimotop group.Experimental models were established by intraluminal occlusion of the middle cerebral artery in rats.Control group and nimotop group were given 30 minutes ischemia then given NS and nimotop(1 mg/kg).The neurological score was investigated and apoptotic cells were detected by TUNEL technique.Results In control group,there are many apoptotic cells.Compared with the control group,the neurological scores decreased(P<0.05),and the number of apoptotic cells reduced significantly(P<0.05).Conclusion Nimotop can reduce apoptosis occurrence and play a role in neuroprotection.更多还原