【摘要】 目的:建立并完善一种新的DNA(cDNA)检测方法,并探讨cDNA检测在鼻咽癌(nasopharyngealcarcinoma,NPC)患者诊断中的应用价值。方法:对196例经病理证实的NPC患者以微量基因组抽提试剂盒抽提血浆DNA,以荧光定量Realtime PCR测定含量,并以健康体检者125例作为对照组。结果:临床分期各组与治疗前相比(P均<0.05)差异有显著性;不论治疗前后,NPC患者各组与正常对照组相比,差异均有,(P均<0.05);cDNA含量与临床分期的关系:根据Ct值的逐渐减少,cDNA含量随着临床分期增加呈增高的趋势,但组间比较并无统计学意义(P>0.05)。结论:利用Realtime PCR法测定NPC患者cDNA,在NPC的诊断、疗效判断有着重要的临床应用价值。更多还原

【Abstract】 Objective:To quantify the circulating DNA in plasma from patients of NPC and evaluate its potential clinical value.Methods:Plasma DNA was extracted by commercial"genomic DNA extraction kit"form 196 patients with NPC and 125 healthy individuals as control and detected by Realtime PCR method.Results:Significant difference (P<0.05) in mean circulating plasma DNA levels between patients of NPC and healthy individuals,and it also has difference significant difference(P<0.05) between untreated patients and posttreatment in difference clinical stage with NPC.The level of circulating DNA was added gradually with the process of clinical stage.However,there was no significant different(P>0.05) between groups in difference clinical stage.Conclusions:Circulating plasma DNA detected by Realtime PCR has potential clinical value in diagnosis and therapeutic effect observing on patients of NPC.更多还原