【摘要】 目的:探讨含表皮生长因子和卵泡抑素结构域的跨膜蛋白（TPEF）、信号转导子和转录激活子1（STAT1）在大肠癌中的表达水平及其意义。方法:采用SYBR Green I荧光定量RT-PCR技术对50例大肠癌组织和相应正常大肠黏膜组织中的TPEFmRNA及STAT1 mRNA的表达进行检测,并分析其表达情况与临床病理资料的相关性。结果:癌组织中TPEFmRNA及STAT1 mRNA的相对表达水平明显低于相应正常组织（P<0.05）;肿瘤TNM分期中T3/T4癌组织表达水平明显低于T1/T2癌组织（P<0.05）;合并淋巴结转移的组织中表达水平亦低于无淋巴结转移者（P<0.05）;TPEFmRNA与STAT1mRNA的表达水平呈正相关(r_癌组织=0.537,r_正常组织=0.851,P<0.001)。结论:TPEF、STAT1基因在大肠癌组织中低表达,与大肠癌的转移相关,可作为大肠癌恶性潜能的检测指标之一。更多还原

【Abstract】 Objective:To investigate the expression levels and significance of transmembrane protein endothelial factor（TPEF） and signal transducer and activaon of transcription 1（STAT1） in colorectal cancer.Methods: Expression levels of TPEF mRNA and STAT1 mRNA were detected by SYBR GreenⅠquantitative real-time PCR in colorectal cancer and corresponding normal mucosa in 50 patients;analysis was performed to study on the correlation between the expression levels of TPEF and STAT1 and clinicopathological parameters,and on the relationship between expression level of TPEF with STAT1.Results:Expression levels of TPEF mRNA and STAT1 mRNA in colocrectal cancer were significantly decreased than that in normal tissue（P<0.05）;and more frequently in stage T1/T2 cancer group than in stage T3/T4 group（P<0.05）;in non-lymph node migrate group were significantly higher than that of the lymph node migrate group（P<0.05）;and there was a positive correlation between the expression level of TPEF mRNA and STAT1 mRNA(r_cancer=0.537,r_normal=0.851,P<0.001).Conclusion:TPEF and STAT1 were lowerly expressed in colorectal cancerous tissues,and highly correlated with invasion and metastasis,may serve as a biomarker for invasiveness and metastasis of colorectal cancer.更多还原