【摘要】 为了选育高产细菌纤维素的木葡糖酸醋杆菌((Gluconacetobacter oboediens)突变菌株,通过硫酸二乙酯和氯化锂复合诱变,得到一株产细菌纤维素能力最高的突变菌株GO2,其细菌纤维素产量为9.91g/L,比出发菌株提高36.5%。同时确定突变菌株GO2的静置培养条件为发酵时间为6d,接种量为8%(v/v),面积/体积比为1.58~2.08cm-1。更多还原

【Abstract】 The bacterial cellulose (BC)-producing strain (Gluconacetobacter oboediens) was mutagenized with diethyl sulphate (DES) and lithium chloride (LiCl), and the mutants of strain GO, namely GO2, was obtained. The BC production by GO2 (9.91 g/L) was about 1.365-fold higher than that of the original strain. The optimum fermentation conditions were determined as follows: fermentation time of 6 days, inoculation volume of 8%(v/v) and area/volume ratio of 1.58-2.08 cm-1.更多还原