【摘要】 本文目的是建立了哥王中西瑞香素含量测定方法,为了哥王的质量评价提供依据。方法是采用HPLC法测定其西瑞香素的含量,色谱柱:AlltechODS(4.6mm×250mm,5μm);流动相:甲醇-0.2%磷酸水溶液(体积比55:45);流速为1.0mL·min-1;检测波长340nm,柱温为35℃;进样量为10μL。结果表明西瑞香素在4.0~20μg范围内与峰面积呈良好的线性关系,其回归方程为Y=4.3662X-7.5398(r=0.9997)。平均回收率为99.98%,RSD=1.70%(n=5)。可见本法具有操作简便准确,回收率好,精密度、重现性高,分析快速的优点,可作为了哥王药材的质量控制方法。更多还原

【Abstract】 A novel method for the determination of the content of daphnoretin in Wikstroemia indica（L.）C.A.Mey （W.indica ） was established in this paper. The content of daphnoretin in W.indica was determined by HPLC on an Alltech C₁₈ （250 mm×4.6 mm, 5μm） column using a mixture of methanol and 0.2% phosphoric acid solution （55:45） as mobile phase with the flow rate was 1.0 mL?min-1 and UV detection wavelength of 340 nm. Column temperature and sample volume were 35 ℃ and 10μL, respectively. Results showed that, within the linear range of 4.0²0 μg, the regression equation was Y=4.3662X-7.5398（r = 0.9997）. The average recovery and RSD of the method were 98.98 % and 1.70 %, respectively. The method was simple, accurate, sensitive and reproducible, which could be used in the quality control of daphnoretin in Wikstroemia indica（L.）C.A.Mey.更多还原