【摘要】 采用分光光度法测定了低温胁迫下两种拟南芥G蛋白突变体的超氧化物歧化酶(SOD)、过氧化物酶(POD)的活性及丙二醛(MDA)含量的变化。结果表明:在低温12h,野生型(Ws)和突变体gpa1-1的SOD酶活性迅速升高,分别为15.501 U/mg protein和9.453 U/mg pro-tein,突变体gpa1-2则在低温24 h升至最高点,酶活性为6.325 U/mg protein。低温胁迫下,三种材料的POD酶活性都呈现降低趋势,而MDA含量呈上升趋势。初步证明外界低温刺激与植物细胞内G蛋白有一定的关联。更多还原

【Abstract】 Enzymatic activities of SOD,POD and content of MDA of two G protein mutants of Arabidopsis thanliana were analyzed by means of spectrophotometer under low temperature stress.The results show that at 12 h of low temperature stress,the activities of SOD rose up in wide type and gpa1-1,respectively were 15.501 U/mg protein and 9.453 U/mg protein,but gpa1-2 mutant rose up at 24 h of low temperature,the activity was 6.325 U/mg protein.Under low temperature stress,the activities of POD from three kind ingredients went down,While the content of MDA rose up.It was preliminarily proved that the relationship must exist between low temperature stress and G protein.更多还原