【摘要】 目的探讨广东汉族单纯性腭裂患者转化生长因子α(TGF-α)基因多态性。方法应用聚合酶链反应-限制性片段长度多态性核苷酸分型技术,以TaqⅠ限制性内切酶消化PCR扩增产物,对43例单纯性腭裂(CPO)患者、68例正常人的TGF-α/TaqⅠ等位基因进行分析。结果单纯性腭裂患者的C2等位基因频率比正常对照组明显增高,差异有统计学意义(P<0.05)。单纯性完全腭裂患者的TGF-α/TaqⅠ基因型与单纯性部分腭裂患者差异无统计学意义(P>0.05)。结论TGF-α基因多态性与广东汉族人的单纯性腭裂的发生有关。更多还原

【Abstract】 Objective To study the Gene polymorphism of transforming growth factor-alpha(TGF-α) in the cleft palate only(CPO) patients.Methods Totally 43 patients with CPO and 68 healthy controls were examined for TGF-α/TaqⅠ genotypes.TGF-α/TaqⅠ typing was carried out by digesting the locus specific polymerase chain reaction amplified products with alleles specific TaqⅠ restriction enzyme(PCR-RELP).Results The C2 allele frequency in patients with CPO was significant higher than that in normal control group.The difference was significant(P<0.05).No significantly difference in the genotype frequency of TGF-α/TaqⅠ among cases with complete cleft palate compared with the other partial cleft palate cases(P>0.05).Conclusion The gene polymorphism of TGF-α is related with the occurrence of nonsyndromic cleft palate only in Han People of Guangdong province.更多还原