【摘要】 目的探讨心肌营养素-1(CT-1)在DCM大鼠心肌的表达及作用。方法30只成年雄性Wistar大鼠随机分为两组:DCM组20只,腹腔内注射阿霉素(ADM)2.5 mg/kg,2次/周,共6周,诱导大鼠DCM模型;对照组10只,同法注射等量生理盐水。第8周时行心脏超声检测,用VG染色观察心肌组织病理改变,用半定量RT-PCR检测CT-1在对照组大鼠与DCM大鼠心肌的表达。结果DCM组的心脏较对照组明显扩大,且心肌纤维化明显,心肌CT-1表达明显升高(P<0.01)。结论在阿霉素诱导的DCM大鼠心肌中CT-1过度表达,可能与其保护心肌细胞、促进心肌细胞肥大有关。更多还原

【Abstract】 Objective We aim to investigate the effect and expression of cardiotrophin-1(CT-1) in the ventricle of rats with dilated cardiomyopathy.Methods Thirty adult male Wistar rats were randomly divided into the control group and the model group.The model group(n=20) was administered adriamycin intraperitoneally at a dose of 2.5mg/kg,twice a week for 6 weeks,and the control group(n=10) was received an equivalent volume of 0.9% saline alone intraperitoneally.Echocardiographic measurements were obtained after 8 weeks.The pathological change was analyzed by histological Van Gieson stain.Later the expressions of CT-1 mRNA were tested with RT-PCR method.Results The hearts of the DCM group showed that the chamber of left ventricle was enlarged and myocardial fibrosis was more obvious than that of the control group;the expression of CT-1 mRNA increased obviously(P<0.01) as well.Conclusion The CT-1 mRNA was over-expression of rats with dilated cardiomyopathy in the adriamycin-induced,which may be relevant to protect myocardial cell and advance myocardial cell hypertrophy.更多还原