【摘要】 目的了解氢醌(HQ)细胞毒性及DNA损伤作用,探讨HQ遗传毒作用的机制。方法用噻唑蓝(MTT)法检测低浓度HQ对中国仓鼠肺成纤维细胞(V79细胞)作用2 h后,细胞的增殖抑制作用;用单细胞凝胶电泳技术(SCGE)检测不同浓度HQ处理V79细胞2 h后DNA的损伤情况,并观察修复2 h和4 h后彗星图像的变化。结果HQ对V79细胞生长产生明显抑制作用,并呈剂量反应关系。单细胞凝胶电泳结果显示,在设置的5个浓度范围内,彗星细胞拖尾率随着浓度的增加而增加,且有统计学意义(P<0.01);在0.405 mmol/L浓度组,尾长、Olive尾矩、彗星矩和尾/头长4个彗星损伤形态学指标随着修复时间的延长而逐渐降低,其差异有统计学意义(P<0.01)。结论在体外培养条件下,低浓度HQ能明显抑制V79细胞的增殖并呈浓度依赖关系,并能引起DNA损伤,主要是单链断裂,这种损伤部分可自身修复。更多还原

【Abstract】 Objective To explore the cell toxicity and DNA damage on V79 cells in vitro caused by hydroquinone.Methods The inhibitory effect of HQ on the viability of cell was evaluated by MTT assay.SCGE was used to detect the DNA damage of V79 cells after exposed to different concentration of HQ for 2 hours.Results As showed in the MTT assay,HQ could obviously inhibit V79 cell proliferation and show concentration-dependent relationship.The comet cells ratio elevated as the concentration increased in SCGE in the range of five concentrations showing significant statistic differences(P<0.01).The four analysis indexes including tail-length,tail/head ratio,TM and CM decreased with the repair time prolonged in the concentration of 0.405 mmol/L.The statistic differences between the repair time of 2 h and 4 h were significant(P<0.01).Conclusions HQ could significantly inhibit V79 cells proliferation showing concentration-dependent relationship.HQ may induce DNA damage,mainly SSB,and the damage could be self-repaired partly.更多还原