【摘要】 目的:研究重度慢性乙型病毒性肝炎患者肠道屏障的变化并初步探讨其临床意义。方法:采用高效液相色谱蒸发光散射器(HPLC-ELSD)检测30例正常人和30例重度慢性乙型病毒性肝炎患者尿乳果糖、甘露醇排泄率比值的变化(L/M);采用酶联免疫吸附法检测上述两组人群血清二胺氧化酶(DAO)的变化,并加以比较。结果:与正常人相比,重度慢性乙型病毒肝炎患者尿乳果糖的排泄率增加(0.065 0±0.027 0 vs 0.086 0±0.022 5,P=0.002),尿甘露醇的排泄率无明显变化,而尿乳果糖/甘露醇排泄率的比值(L/M)明显升高(0.031 6±0.013 4 vs 0.043 0±0.019 8,P=0.011)。而重度慢性乙型病毒肝炎患者血清DAO的水平与正常相比也明显升高(6.654 7±2.221 7 vs 10.981 3±2.973 4,P<0.000 1)。结论:重度慢性乙型病毒肝炎患者肠黏膜通透性和血清DAO均明显增高,提示重度慢性乙型病毒性肝炎患者存在肠黏膜屏障的损伤。更多还原

【Abstract】 Objective:To study the change of intestinal mucosa barrier in heavy degree chronic hepatitis B patients. Methods:Thirty cases normal human and 30 cases heavy degree chronic hepatitis B patients were measured the change of intestinal permeability by u- rine lactulos/ mannitol ratio(L/M),respectively.The levels of lactulos and mannitol in the urine were detected by high performance liquid chromatography with evaporative light scattering detector(HPLC-ELSD). The serum diamine oxidase(DAO) was measured by ELISA.Results:As compared with normal control,the level of lactulos in urine in heavy degree chronic hepatitis B patients was significant raised (0.065 0±0.027 0 vs 0.086 0±0.022 5,P =0.002),but the level of mannitol in urine was unchanged.A significant rise in urinary L/M ratios was found in heavy degree chronic hepatitis B patients as compared with normal control(0.031 5±0.013 4 vs 0.043 0±0.019 8,P =0.011).Meanwhile,as compared with normal control,the serum level of DAO in heavy degree chronic hepatitis B patients was raised significantly (6.654 7±2.221 7 vs 10.981 3±2.973 4,P<0.000 1).Conclusion:The serum levels of DAO and the intestinal permeability were significantly higher in heavy degree chronic hepatitis B patients than in normal controls.These results indicate that there was an intestinal mucosa barrier damage in heavy degree chronic hepatitis B patients.更多还原