【摘要】 以带1～3个叶原基、不同大小的玉林大蒜鳞茎尖为外植体,在附加不同激素配比(1.0～2.0mg/L 6-BA、0.1～0.5mg/L NAA)的MS和B5培养基上进行培养,结果表明:芽初代诱导的适宜培养基为B5+1.5mg/L 6-BA+0.5mg/L NAA,出芽率为96%;芽增殖的适宜培养基为B5+0.5mg/L 6-BA+0.05mg/L NAA,增殖倍数为4.5,增殖效果最佳。ELISA法病毒检测结果表明,选取剥离0.5～0.6mm带有1～2个叶原基的茎尖进行培养,试管苗脱毒率达90%以上,脱毒效果明显。更多还原

【Abstract】 The present study has been conducted to develop a virus-free propagation technology for growing Yulin garlic.Shoot tips of Yulin garlic plants with 1-3 leaf primordia were used as explants and cultured using MS or B5 basic medium with different hormone concentrations(1.0-2.0 mg/L 6-BA,0.1-0.5 mg/L NAA).The results showed that the optimal medium for the induction of adventitious buds was B5+1.5mg/L 6-BA+0.5mg/L NAA,which recorded 96% ad-ventitious buds induction rate.The optimal medium for bud multiplication was B5+0.5mg/L 6-BA + 0.05mg/L NAA,where the multiplication multiple reached 4.5 times.The ELISA results showed that the virus-free rate of shoot tip was over 90%,when shoot tip was 0.5-0.6mm and with 1-2 leaf primordia.更多还原