【摘要】 目的建立同时测定金银花及金芪降糖片中绿原酸的反相高效液相色谱法。方法色谱柱为Diamondsil C18柱(250mm×4.6mm,5μm),以乙腈-0.4%磷酸溶液(13∶87)为流动相,检测波长为327nm,体积流量为1.0mL/min。结果在上述条件下测定金银花中绿原酸为2.49%,金芪降糖片中绿原酸为每片8.776mg。结论本方法操作简单、结果准确、回收率高、重现性好,可用于金银花及金芪降糖片的质量控制。更多还原

【Abstract】 Objective To establish a reversed-phase HPLC(RP-HPLC)method for the determination of chlorogenic acid content in Flos lonicerae and Jinqi Jiangtang Tablet.Methods The separation of chlorogenic acid was carried out by using the Diamondsil C18 column(250 mm×4.6 mm,5 μm)with the mobile phase containing acetonitrile-0.4% orthophosphoric acid(13∶87)and a detective wavelength at 327 nm.The flow rate was 1.0 mL/min.Results The contents of chlorogenic acid in Flos lonicerae and Jinqi Jiangtang Tablet were 2.49% and 8.776 mg/t,respectively.Conclusion The method is simple,accurate with good recovery and repeatability.It can be used to control the quality of Flos lonicerae and Jinqi Jiangtang Tablet.更多还原