【摘要】 MicroRNAs(miRNAs)是一类重要的调节基因转录的非编码小RNA。其中miR-1、miR-133、miR-206是肌肉特异表达的microRNA,同属miR-1家族。它们在胚胎发育时期的表达及作用已有很多文章阐述,但很少有文章详细分析它们在成体中的表达。为检测这3类microRNA的表达差异,选择BALB/c品系小鼠的股二头肌,提取RNA。设计microRNA专用的茎环状引物,通过实时定量PCR,检验miR-1、miR-133、miR-206和miR-181a在骨骼肌组织中的表达情况。结果发现,miR-133的表达量要明显高于miR-1和miR-206,miR-1略高于miR-206的表达量,作为对照,miR-181a的表达量非常微小。分析该现象,根据资料和网络软件预测,绘制这3类microRNA对肌肉生长发育作用的关系图,初步解释各种microRNA之间呈现表达差异的原因。为下一步研究成体中microRNA及其靶位点提供依据和方向。更多还原

【Abstract】 MicroRNAs(miRNAs) are small non-coding RNAs emerging as important post-transcriptional gene regulators.miR-1,miR-133,miR-206 are the miRNAs specifically expressed in skeletal muscle tissue,they all belong to miR-1 family,but divide into miR-1/miR-206 and miR-133a/b.The expression and the roles of them in embryogenesis have been expounded by many literatures,but few article refers to the expression of them in adult.In this study,examined the expression of miR-1,miR-133 and miR-206,the miRNAs specifically expressed in skeletal muscle tissue,on the biceps flexor cruris of BALB/c mouse by Real-time PCR,and miR-181a is as the control.The expression of miR-133 is much more than that of the miR-1 and miR-206;the expression of miR-1 is a little more than miR-206’s when the miR-181a is little expression.To find the reason of this phenomenon,the map of the relation between the microRNA and the growing of muscle was protract by literature and network forecast.This result provides the evidence and the direction for the following research of microRNA and target gene in adult.更多还原