【摘要】 本研究运用分子生物学手段探究了一氧化氮(NO)在猪早期胚胎发育过程中的表达情况及其相关的规律。应用RT-PCR方法将猪各发育阶段的早期胚胎的内皮型一氧化氮合酶(eNOS)和诱导型一氧化氮合酶(iNOS)进行检测,然后对产物进行半定量分析。结果表明,iNOS在猪早期胚胎发育的整个过程中都有表达,其相对表达量随着胚胎发育进程呈现上升的趋势,在桑葚/囊胚阶段达到最高;eNOS仅仅是在2-细胞期和4-细胞期有表达,其相对表达量在2~4-细胞期间的差异不明显;在猪2~4-细胞胚胎发育过程中,iNOS的相对表达量高于eNOS的相对表达量。结果表明,在猪早期胚胎发育中NO的产量主要由iNOS调节。更多还原

【Abstract】 In this study,mRNA isolated from embryos was subjected to reverse transcription polymerase chain reaction to investigate the expression of eNOS,iNOS mRNA in normal pathenogenetic activation porcine embryos.The results showed that eNOS was expressed only in two-cell and four-cell stage embryos,while iNOS was expressed around early embryo development stages.These studies determined that inducible NOS(iNOS) and endothelial NOS(eNOS) were differentially distributed in cells along the development,higher level of eNOS was detected at four-cell stage embryo but iNOS at morula/blastocyst stage.The study provides the first evidence for the presence of mRNA of NOS isoforms during porcine embryogenesis.Furthermore,the expression of NOS during the embryo development suggest that NO may be crucial importance for reproductive events that occur in this period.更多还原