【摘要】 目的探讨乙肝病毒表面抗体定性测定S/CO（标本吸光度/临界值）值与定量测定值的相关性。方法时间分辨荧光免疫分析法（TRF）及酶联免疫吸附试验（ELISA）检测不同浓度的血清,绘制标准曲线,建立方程。结果S/CO值与定量测定值呈曲线正相关,转换方程式为Y=8.911 8×e^{0.177 4X}。S/CO计算值与实测值组内相关系数为0.934。结论乙肝病毒表面抗体含量可通过定性S/CO值推算,适用于基层未开展乙肝标志物定量检测的实验室应用。更多还原

【Abstract】 Objective To study the relativity of S/CO qualitative determination of hepatitis B surface antibody and quantitative assay.Methods The different concentrations of sera were determined with time-resolved fluorescence immunoassay and enzyme-linked immunosorbent assay.The standard curve was drawn and the equation was established.Results In the curve,S/CO level was positively correlative to the quantitative detection value,and the conversion equation was Y=8.9118×e^0.1774X The intraclass correlation coefficient of S/CO calculating level and quantitative value was 0.934.Conclusion The content of hepatitis B surface antibody can be estimated by S/CO value.It can be used in basic-level laboratories which don’t undertake the quantitative determination of HBV markers.更多还原