【摘要】 目的研究牡芍合剂对骨折术后疼痛模型大鼠丘脑中β-内啡肽(β-EP)及八肽胆囊收缩素(CCK-8)影响的时间依赖性,探讨牡芍合剂术后应用的时间。方法选取体质量250～300 g SPF级雄性Wistar大鼠随机分为:正常对照组(空白组)、模型组、牡芍合剂治疗组和曲马多对照组。采用Brennan术后痛模型,术后曲马多对照组、治疗组立即给药,给药后分别于1 h、24 h、48 h处死动物取脊髓,采用放射免疫法测定β-EP及CCK-8的含量。结果与模型组相比,牡芍合剂能显著升高术后疼痛模型大鼠丘脑中β-EP含量,且随着用药时间的增加其促进β-内啡肽释放的作用逐渐加强;同时它可降低CCK-8的含量,此作用大小与用药时间无关。结论牡芍合剂可以促进β-EP的分泌,且有时间依赖性;同时牡芍合剂可以抑制CCK-8的分泌,无时间依赖性。更多还原

【Abstract】 Objective To study the chronergy of effect of Mushao composition onβ-endorphin (β-EP)and cholecystokinin-octopeptide(CCK-8)in the thalamencephals of rat models of bone fracture with postoperative ache,and to explore the opportunity of Musbao composition administration after fracture operation.Methods Ninety-six healthy male Wistar rats of SPF level weighing 250-300 g were randomly divided into healthy control group(blank group),model group,therapy group and tramadol control group.The rat model of Brennan postoperative ache was developed.The corresponding agent was given immediately after operation in therapy group and tramadol control group.The animals were sacrificed so as to collect the spinal cord samples at 1 h,24 h,48 h timepoint after operation. The contents ofβ-EP and CCK-8 were measured by applying radioimmunoassay.Results Compared with model group,Mushao composition significantly increased the level of theβ-EP in the thalamencephal, and the content ofβ-EP was increased with time;while it could decrease the level of CCK-8, which wasn’t associated with the time of medication.Conclusion Mushao composition can increase the content ofβ-EP in time-depedent manner,and while it can inhibit the secretion of CCK-8,which isn’t associated with the time of medication.更多还原