【摘要】 首次通过激光诱导荧光光谱技术(LIF)研究运动训练大鼠心脏、肾脏、肝脏、脂肪以及前腿肌和后腿的比目鱼肌和腓肠肌的自体荧光光谱特性。测量所用的激发光波长为250～650nm而发射波长为300～700nm。比较参照组和3组不同运动状态组的三维荧光光谱,主要在腓肠肌的光谱中发现了和运动相关且位于激发波长(340±10)nm和发射波长(460±10)nm区域的特有荧光峰。根据这一荧光峰可以判别其对应的荧光物质是NADH(还原态烟酰胺腺嘌呤二核苷酸)。比较3组不同运动模式组的荧光光谱,发现运动模式与其峰强具有明显的相关性。研究结果表明,运动大鼠腓肠肌的能量代谢强于前腿、比目鱼肌及其他脏器,且NADH自体荧光光谱特性是判断肌肉代谢程度的有效的指标之一。更多还原

【Abstract】 The laser-inducted fluorescence spectrum technology(LIF) was used for the first time to study the autofluorescence spectral characteristics of the heart,kidney,liver,fat,foreleg muscle,hind leg soleus muscle and musculus gastrocnemius of the rat performing motion exercises.The wavelength of the excitation light used during the measurement was in the range of 250-650 nm and the emission wavelength was 300-700 nm.When comparing the three-dimensional fluorescence spectra of the control group with those of the four groups of different motion states,a specific fluorescence peak related to the motion and located in the area where the excitation wavelength was(340±10) nm and the emission wavelength was(460±10) nm was found mainly in the spectra of the soleus muscle.From this fluorescence peak,it is possible to determine that its corresponding fluorescent substance is NADH(nicotinamide adenine dinucleotide reduced).When comparing the fluorescence spectra of the four groups of different motion modes,it was found that the motion mode has a conspicuous relativity with the peak intensity.The results show that the energy metabolism of the soleus muscle of the rat in motion is stronger than that of the foreleg,soleus muscle and other visceras,and the autofluorescence spectral characteristics of NADH form one of the effective indexes for determining the muscular metabolism degree.更多还原