【摘要】 金属硫蛋白(MT)是一种低分子量、高巯基含量、能结合金属离子的功能性蛋白,将MT基因转入蘑菇,可以获得具有重要生理功能的蘑菇以便为保健食品生产提供原料,但对MT蘑菇的富锌能力缺乏系统研究。文章报道了用电感耦合等离子体-原子发射光谱(ICP-AES)法对富锌培养中的MT侧耳(Pleurotusostreatus)菌丝和未转化的侧耳菌丝(对照)锌含量进行了测定,分析了MT侧耳菌丝的耐锌能力、富锌特性和锌有机化程度。结果发现转MT侧耳在锌浓度0~1.2 mmol.L-1范围内菌丝生长速度最高,超过1.6mmol.L-1时菌丝生长开始受到抑制;转MT侧耳有较强的富锌能力,当培养液中锌质量浓度为0.6~1.2mmol.L-1时,菌丝体中锌含量在2.56~27.49 mg.kg-1之间,与锌添加量呈正相关,且此浓度范围内菌丝体的生长情况良好;在锌浓度为0.6 mmol.L-1的液体培养基中,第7 d转MT侧耳菌丝锌有机化程度为88.7%,比对照(82.1%)显著提高(α=0.05),说明转入侧耳的MT有利于无机锌向有机锌转化;对在不同浓度下培养7 d的两种菌丝测定发现,其有机化程度差异不显著,说明锌与菌体内的物质已经紧密结合形成有机锌复合物,锌浓度对锌的吸收量有影响但对锌的有机化程度影响不大。更多还原

【Abstract】 Metallothioneins(MTs) are a family of low molecular weight,cysteine-rich and metal-binding functional proteins.Transgenic MT mushroom can be used as functional food additives,but its zinc-enriching ability has not been studied systemically until now.The zinc contents in mycelia of transgenic MT mushroom(Pleurotus ostreatus) and wild type mushroom mycelia cultivated in different zinc concentration media were analyzed by ICP-OES.The growth status,zinc-enriching ability and degree of zinc in organic form(DZOF) were also analyzed.Results showed that MT mushroom mycelia grew rapidly,but the growth was inhibited when the zinc content in solid media was higher than 1.6 mmol·L-1.MT mushroom mycelia could enrich more zinc than that of wild type,and the zinc content in MT mushroom mycelia could be 2.56-27.49 mg·kg-1 when it was cultivated in a liquid media with 0.6-1.2 mmol·L-1 zinc.DZOF of MT mushroom mycelia in a liquid media with 0.6 mmol·L-1 zinc at 7 d was significantly higher(88.7%) than that in the wild type(82.1%,α=0.05),but there was no significant difference in DZOF when the MT mushroom mycelia was cultivated in a liquid media with different zinc content at 7 d.更多还原