【摘要】 利用傅里叶变换红外光谱(FTIR)法测定了2个不同耐铝性大豆品种在不同浓度铝处理下根、茎、叶的红外光谱。通过对各谱图的比较分析发现,除928~1200 cm-1区域的FTIR差异比较明显外,未经处理的2个品种大豆红外光谱基本相似。而铝处理后2个品种大豆根、茎、叶的红外吸收峰形及峰值均与对照有较大的区别,且随铝浓度升高其FTIR区别越为明显。此外,结果表明浙春3号(铝敏感品种)的变化幅度比浙春2号(铝耐性品种)更为明显,这说明铝敏感大豆在铝胁迫下物质代谢受到了较大的影响。因此,采用FTIR法鉴别大豆不同耐铝性能够提供较为准确的结果,具有广泛的应用前景。更多还原

【Abstract】 The spectra of root,stem,leaf of soybean samples with 0,20,40,60 mg·L-1 Al 3+ were determined by Fourier transform infrared(FTIR) spectrometry with OMNI-sampler.Little difference was found in the spectra of leaf between two soybean cultivars,aluminum-resistant cultivar Zhechun NO.2 and aluminum-sensitive cultivar Zhechun NO.3,except the indices of wave number-absorbance from 928 to 1 200 cm-1,and similar results were also observed in stem and root samples of the two soybean cultivars with 0 mg·L-1 Al 3+.However,results from the comparison of the spectra showed some distinguishable differences in the intensity and the shape of absorption peaks of their FTIR spectra from 721 to 3 366 cm-1 of Al-stressed samples and control samples between the two soybean cultivars,and more evident differences of FTIR were exhibited in Al-stressed roots,stems and leaves with higher concentration of Al 3+.The increased absorbance at 2 929 and 3 350 cm -1 was found in root FTIR spectra with 20,40,60 mg·L-1 Al 3+, while roots got maximum absorbance at wave number of 1 375 cm-1 with 20 mg·L-1 Al 3+,which decreased with higher concentration of Al 3+,and the same results were showed at wave numbers of 1 410,1 423,1 549 and 1 645 cm-1.Absorption peak showed maximum at wave numbers of 1 051,2 850,2 929 and 3 350 cm-1 in stem FTIR spectra with 60 mg·L-1 Al 3+ .There was little difference between the spectra of Al-stressed leaves and controls at wave numbers from 1 750 to 2 750 cm-1,but visible difference in leaf spectra was exhibited at other wave number.Moreover,the results showed that the FTIR spectra of aluminum-sensitive cultivar Zhechun NO.3 showed much more observable differences than aluminum-resistant cultivar Zhechun NO.2 with different concentration of Al,implying that the material metaboly of aluminum-sensitive soybean was evidently affected by Al.Therefore,FTIR spectra could be used broadly for identification of the endurance of different soybean cultivars to Al.更多还原