【摘要】 利用ISSR分子标记对来自10个不同生态地区的红锥优良种质进行基因组遗传多样性分析.从20条引物中筛选出11条具有扩增多态性的引物用于ISSR-PCR扩增,共扩增出435条带,其中多态性条带325条,占总扩增条带的74.7%,平均每个引物扩增3.95条.ISSR标记分析揭示出红锥10个优良种质的遗传相似系数介于0.473 7~0.808 1之间,平均值为0.728 7.用STATIS统计软件的欧氏可变类平均法构建类型之间的分子系统树,聚类分析结果将10个优良红锥种质分为4个类群.更多还原

【Abstract】 Genetic diversity of fine germplasm resources of Castanopsis hystrix from ten different ecological regions was analyzed by using the inter-simple sequence repeat(ISSR),and 11 effective primers were selected from 20 primers.The result showed as follows: 435 bands were amplified,among which 325 bands were polymorphic,and percentage of polymorphic bands were 74.7 %,an average of approximately 3.95 bands per primer.Genetic similarity indexes of ten germplasms of C.hystrix ranged from 0.473 7 to 0.8081,and the average value was 0.728 7.Static software was used to calculate the Nei’s genetic distance and a dendrogram was constructed based on UPGMA cluster analysis.These ten germplasms were divided into 4 major groups,and their genetic relative and the genetic distance were discussed.更多还原