【摘要】 B-藻红蛋白是一种理想的生物光电材料.利用静电组装技术制备了聚二甲基二烯丙基氯化铵(PDDA)与B-藻红蛋白组装的多层复合薄膜.对薄膜的可见吸收光谱和荧光光谱进行了分析,结果表明:与B-藻红蛋白在溶液中的光谱特征相比,B-藻红蛋白在薄膜上的最大吸收峰由545 nm红移至553 nm,并在573 nm处产生了延迟荧光现象,且薄膜的最大吸光度和光致发射强度均与组装层数呈线性递增关系.随着薄膜组装层数的增加,倒置荧光显微镜能很好地观察到B-藻红蛋白在薄膜上出现明显的聚集体.更多还原

【Abstract】 B-Phycoerythrin was an novel bio-photoelectronic material.Multilayer films formed of PDAA and B-PE were prepared by electrostatic self-assembly method.Visible absorption spectrum and fluorescent spectrum of the multilayer films were studied.The result showed the maximum absorption peak occurred red shift from 545 nm to 553 nm,and a delayed fluorescence peak at 573 nm was observed,the maximum absorption and photoluminescence intensity increases in proportion to the assembled layers.With the increase of the self-assembly layers,Obvious aggregate of B-PE was observed on the film by Inverted Microscope Eclipse.更多还原