【摘要】 目的:研究端粒酶抑制剂IFN-α2b对人葡萄膜黑色素瘤细胞端粒酶活性的抑制作用。方法:采用原代培养的人葡萄膜黑色素瘤细胞,用特定时间下不同浓度以及特定浓度下不同时间的端粒酶抑制剂IFN-α2b作用于体外培养的黑色素瘤细胞,并设立空白对照组。采用PCR-ELISA法及PCR-PAGE法测定细胞中端粒酶活性的变化,采用单因素方差进行统计分析。结果:随着IFN-α2b浓度的增加及作用时间的延长,端粒酶的活性出现逐步的下降,在药物浓度达到50kU/L以及作用时间达到24h后,出现明显的抑制作用,并在500kU/L及48h达到抑制高峰。结论:IFN-α2b可有效降低体外培养的人葡萄膜黑色素瘤细胞的端粒酶活性,并呈浓度和时间依赖性。更多还原

【Abstract】 AIM:To study the restrain effects of telomerase inhibitor IFN-α 2b(Interferon-α 2b)on the telomerase activity to uveal melanoma cells of human eyes..METHODS:Using IFN-α 2b individually with different concentration and time acted on the primary cultured uveal melanoma cells of human eyes and compared with the controls.The changes of telomerase activity were observed by PCR-ELISA and PCR-PAGE.The restrain results were compared with a one-way analysis of variance(ANOVA)..RESULTS:The tolemerase activity depressed as the increasing concentration and time of IFN-α 2b.Significant inhibition appear at the concentration attained 50kU/L or the time attained 24 hours.At the point which 500 kU/L or the time attained 48 hours the tolemerase activity depressed very obviously..CONCLUSION:As an effective telomerase inhibitor,IFN-α 2b can depress the tolemerase activity of uveal melanoma cells effectively.And it appeared concentration and time dependency.更多还原