【摘要】 目的:观察雷公藤多甙(tripterygiitotorum,TⅡ)及FK506滴眼液局部应用对大鼠角膜移植免疫排斥反应的影响。方法:建立大鼠角膜移植模型,随机按局部滴用药物分组成A,B,C组,用裂隙灯记录及比较各组移植排斥指数,并比较角膜排斥发生时间。分别于术后15d及30d行常规病理学检查。并通过免疫组化检查CD25,CD5+4在角膜植片基质中的表达。结果:0.3g/LTⅡ及0.5g/LFK506抑制角膜移植免疫排斥反应的效应均有效,两者相比效果无显著性差异。HE染色及免疫组化分析可见15d时各有效组角膜植片厚度基本正常,基质中仅有少量CD25,CD5+4阳性细胞表达、淋巴细胞浸润,新生血管少见。结论:TⅡ与FK506疗效近似。具有抑制高危角膜移植免疫排斥反应发生的临床应用价值。更多还原

【Abstract】 AIM:To observe and compare the effect of the TⅡ,FK506 ophthalmic solutions on the corneal allograft rejection.·METHODS:The corneal transplantation model on the closed colony rats were set up and then were randomly divided into A,B and C groups by the using of different eye-drops.Graft fate was assessed clinically by slit-lamp biomicroscope examination,included the corneal clarity,edema and vascularization,and the rejection time.Respectively,after 15 days and 30 days of routine histopathological examination.And inspect expression by immunohistochemistry CD25,CD+54 in the corneal graft stroma.·RESULTS:There were no statistical different between group 0.3g/L TII and 0.5g/L FK506 in prolonging the corneal survival.While comparing the histopathological and immunohistochemistry findings between the groups,the results confirmed that the topical application of TⅡ and FK506 eye-drops could significantly reduce the inflammatory cells infiltrating the graft stroma and the neovascularization,whilst reduce the production of ICAM-1 and IL-2.·CONCLUSION:The corneal allograft rejection could be significantly suppressed by the local use of TII ophthalmic solution.0.3g/L TII has the same effect as the 0.5g/L FK506 in prolonging the corneal survival.更多还原