【摘要】 目的观察pcDNA3-hBMP2转染兔骨髓基质细胞前后的生长特性变化,为骨组织工程的种子细胞选择打下基础。方法抽取成年雄性新西兰大白兔髂骨骨髓,密度梯度离心获得骨髓基质细胞,培养传至第5代,按处理方法分为常规培养液组(A组),pcDNA3-hBMP2转染组(B组)。观察原代MSC及第5代的细胞形态等生长特性并与转染组进行比较。结果原代MSCs为短梭形,簇形生长,传代细胞呈长梭形,旋涡形生长。转染组细胞72 h后表达BMP2,瞬间表达为100%。转染细胞未经筛选培养4周细胞形态发生较明显地变化,细胞生长周期改变。结论pcDNA3-hBMP2成功转染兔骨髓基质细胞并改变其生物学特性。更多还原

【Abstract】 Objective To observe main biological characteristic and changes before and after MSCs were transfected with pcDNA3-hBMP2.Methods MSCs from an adult New Zealand white rabbit were isolated and cultivated.MSCs were divided into two groups.Group A was transfected with pcDNA3-hBMP2 and group B was not transfected as control group.Immunohistochemical staining was used for detecting BMP2 expressing 2 days after transfection.The cell morphological changes,cell divide index and cell cycle were observed after transfection.Results Rabbit MSCs could be successfully transfected by pcDNA3-hBMP2 through medium fugene 6 and express BMP2.Primary MSCs proliferated as short spindle shape,while post-transfected MSCs showed triangle-shape,and transfected-cells had significant difference in cell cycle and cell divide index.Conclusion Rabbit MSCs can be successfully transfected by pcDNA3-hBMP2 and after transfection many biological characteristics are significantly changed in this study.更多还原