【摘要】 采用原子力显微镜(AFM)研究了聚乳酸(PLLA)表面Ⅰ型胶原纳米图案化的过程。发现吸附层的形态变化与干燥条件及PLLA表面的疏水性有关。经氮气快速吹干形成的吸附层较为致密;而保持较高湿度并维持较长水膜浸润时间,由于去湿作用的影响,形成纤维网状纳米图案化结构。此结构受浸润干燥时间、胶原浓度、基底疏水性等因素的影响,随浸润干燥时间延长,致密的胶原膜破裂,逐渐形成纳米图案化结构,胶原浓度由0.01mg/mL增加到1mg/mL均产生类似现象,且网状结构趋于紧密,PLLA分子量不同,胶原吸附层形成不同的结构形态,显示PLLA分子量对胶原的结构具有调控作用。更多还原

【Abstract】 Adsorption of typeⅠcollagen on poly(L-lactide acid)(PLLA) substrates was studied by atomic force microscopy (AFM). The result showed that tightly collagen layers was obtained,on the one hand,by fast drying under a nitrogen flow and nano-patterned structure was obtained by slow drying under high humidity and long drying duration for dewetting occurred to the liquid-substrate interface. The discontinuous patterned structure was regulated by drying duration,concentration of collagen solution and the hydrophobicity of substrate. With increasing drying duration,continuous collagen layer ruptured and nano-patterned structure formed ;and With increasing concentration of collagen layer,the density of the net-like structure increased. The hydrophobicity of substrates differed with the molecular weight of PLLA. The greater w was,the stronger the hydrophobicity and the effect of dewetting. As a result,the adsorbed collagen structure changes and shows the regulation ability of molecular weight on it.更多还原