【摘要】 目的了解湛江地区无偿献血员中HTLV-I感染情况。方法采用ELISA法检测7 630名湛江地区无偿献血者血清中HTLV抗体,其中1 896名为外地人群,5 734名为本地人群。阳性者进行双孔复检,仍阳性者采用PCR法进行确证及系统发生树分析。结果ELISA法检测1 896名外地人群HTLV-I抗体阴性;5 734名本地无偿献血者中发现1例HTLV-I感染,PCR确证仍为阳性,阳性率为0.17‰。获得1株同属世界群世界人种亚型的HTLV-I毒株的部分核酸序列。结论建议在本地区无偿献血员中开展HTLV-I常规筛查。更多还原

【Abstract】 Objective To study the seroprevalence of HTLV-I in voluntary blood donors in Zhanjiang district.Methods Serum HTLV-I antibody was detected by ELISA in 7 630 voluntary blood donors including 1 896 outlanders and 5 734 natives in Zhanjiang district.The positive samples were reexamined and then confirmed by PCR and phylogenetic analysis.Results Only one(0.17‰)of 5 734 native donors was seropositive for HTLV-I by ELISA and confirmed by PCR,while all outlanders were seronegative.Phylogenetic analysis showed that HTLV-I belonged to the subtype of cosmopolitan group.Conclusion It is suggested that the routine screening for serum antibody to HTLV-I should be performed in voluntary blood donors in Zhanjiang district.更多还原