【摘要】 目的测定13个不同产地布渣叶样品中总黄酮含量及其清除自由基活性。方法采用Al(NO3)3-NaNO2-NaOH体系比色法测定总黄酮含量,采用1,1-二苯基-2-苦基肼自由基(DPPH.)、羟自由基(.OH)和超氧自由基(O2.-)等模型测定布渣叶总黄酮清除自由基活性。结果各产地总黄酮含量差异较大,其中以广西来宾样品含量最高,达到了55.22 mg.g-1,平均为27.06 mg.g-1。各产地总黄酮样品均具有清除DPPH.活性,其自由基清除率随供试品总黄酮浓度的增大而提高,呈现一定的量效关系;但其清除.OH和O2.-活性与总黄酮浓度关系不明显。结论不同产地布渣叶总黄酮含量差异较大;各产地布渣叶总黄酮均具有较好的自由基清除活性。更多还原

【Abstract】 Objective To analyze the contents of total flavonoids in Microcos paniculata L.from different habitats and the radical-scavenging activity of total flavonoids.Methods Contents of total flavonoids in the extracts were analyzed by spectrophotometry based on Al(NO3)3-NaNO2-NaOH reaction system,and the radical-scavenging activity towards 1,1-diphenyl-2-picryhydrazyl(DPPH·) radical,hydroxyl radical(·OH)and superoxide anion radical(O2-·),were determined in vitro.Results Contents of total flavonoids in Microcos paniculata L.varied with habitats and 55.22 mg·g-1 was the highest.The average content was 27.06 mg·g-1.All the samples had radical-scavenging activity against DPPH·,·OH and O2-·.There was a clear dose-effect relationship between the radical-scavenging activity against DPPH· and the concentration of flavonoids,but no this relationship was found when against·OH and O2-·.Conclusion Contents of total flavonoids in Microcos paniculata L.varied with habitats,and all the plants have radical-scavenging activity.更多还原