【摘要】 目的建立高效液相色谱法测定复方益母口服液中水苏碱的含量。方法以Kromasil C18(5μm,250mm×4.6 mm)为色谱柱,乙腈-0.05 mol.L-1磷酸二氢钾溶液(体积比18∶82,pH值5.5)为流动相,流速1.0 mL.min-1,柱温35℃,检测波长215 nm,以外标法进行检测。结果水苏碱进样量在1.00~25.00μg范围内具有良好的线性关系,r=0.999 7,样品平均加样回收率为98.1%(n=9),RSD为1.17%。结论本方法简便,结果准确可靠,重复性好,可作为控制该制剂质量的方法。更多还原

【Abstract】 Objective To establish an HPLC method for the determination of stachydrine in complex Yimu oral liquid.Methods Samples were analyzed on a Kromasil C18 column(5 μm,250 mm×4.6 mm) using CH3CN-0.05 mol/L KDP(18∶82,pH 5.5) as mobile phase under the flow-rate of 1.0 mL/min.Oven temperature was 35 ℃,and the detection wavelength was 215 nm.Results Stachydrine was well separated from other impurities.The method was validated for a linear range of 1.00-25.00 μg for stachydrine(r=0.999 7).The average recovery of stachydrine was 98.1%(n=9) with a relative standard deviation of 1.17%.Conclusion The method is simple,convenient,reliable and sensitive,which could be used for the quality control of complex Yimu oral liquid.更多还原