【摘要】 通过分析不同给药剂量硝酸钕[Nd(NO3)3][2,10,50 mg/kg(体重)]后,雄性Wistar大鼠完整肝组织的MAS1HNMR谱和肝组织提取物的1HNMR谱,结合肝组织病理切片图,研究了稀土化合物Nd(NO3)3在大鼠体内的急性生物效应.利用模式识别方法对给药Nd(NO3)3组和对照组大鼠肝组织1H NMR谱图数据进行了分析.结果表明,腹腔注射Nd(NO3)3后,大鼠肝脏中甘油三酯、亮氨酸(异亮氨酸)、乳酸、丙氨酸、丙酮酸、磷酸胆碱和葡萄糖含量升高,氮氧三甲胺含量降低.肝脏病理图显示,50 mg/kg(体重)组大鼠肝细胞可见微小坏死灶和门管区炎细胞轻度增多.推测硝酸钕能影响大鼠肝脏中能量代谢(糖代谢和脂肪代谢)和氨基酸代谢,对大鼠肝脏造成损伤,且其损伤程度随剂量的增加有增强趋势.更多还原

【Abstract】 The biochemical effects of neodymium nitrate were studied by NMR spectroscopic-based metabonomic approach.Male Wistar rats were treated intraperitoneally with different doses(2,10 and 50 mg/kg body weight) of Nd(NO3)3.Liver samples from Nd3+-treated rats were analyzed either by 600 MHz 1H MAS NMR techniques for intact liver or using high resolution(liquid state) 1H NMR for liver chloroform/water/methanol extracts.All NMR spectra data were analyzed by pattern recognition using principal components analysis.Liver lesions induced by Nd(NO3)3 were represented by increased triglyceride,leucine(isoleucine),lactate,alanine,pyruvate,phosphocholine and glucose concentration,and reduced trimethylamine N-oxide(TMAO) concentration in liver.Histopathology examination showed cell necrosis and inflammation in the liver of 50 mg/kg body weight dosed rats.It was demonstrated that neodymium nitrate could cause damages to rats liver by disturbing the energy metabolism(glucose and lipid metabolism) and amino acid metabolism in the liver.更多还原