【摘要】 利用共价固定方法将抗血清白蛋白固定到硅烷化玻璃基底上,并通过摩擦其表面形成5CB液晶整齐均一取向排列的基底.考察了不同浓度的人血清白蛋白与基底作用后液晶在基底上形成的偏光光学图像的差异,并利用自行提出的"图像加权平均灰度值"定量分析了图像灰度与人血清白蛋白浓度的关系.对比研究了基底上的特异性吸附与非特异性吸附引起的液晶偏光光学图像的差异以及调制偏振光能力,结果表明,该基底具有很高的特异性.该方法可望发展成为一种灵敏、非标记的光学免疫检测方法.更多还原

【Abstract】 The uniform orientation of liquid crystal molecule can be changed easily when exposure to different biological environment and thus lead to the changes of optical images obtained from crossed polarized light with liquid crystal sandwiched between polarizer and analyzer.Based on this principle,we report the uniform planar orientation of thermotropic liquid crystals,4-biphenyl-4′-pentyl-carbonitrile(5CB),on the films of anti-human serum albumin(anti-HSA) covalently immobilized on the surface of glass microscope slides using(3-aminopropyl)triethoxysilane(3-APTES) and glutaraldehyde(GA) and mechanically rubbed using a rubbing machine.Following immersion of these rubbed films of anti-HSA into aqueous solutions containing different concentrations of HSA,we observed the differences of images and quantificationally analyzed the relationship between gray scale of images and the concentration of HSA using the concept of weighted average of gray-scale value of image.Following immersion of these rubbed films of anti-HSA into solutions containing HSA,BSA,fibrinogen,lysozyme,we compared specific adsorption with nonspecific adsorption on these rubbed films of anti-HSA by observed the differences of images and modulation of polarized light.The result suggests that these types of substrates(rubbed films of proteins) possess high specificity.The provided methods have the potential to be useful in a variety of sensitive,label-free biomolecular detection where specific binding of a target species to its antibody(or antigen) can be imaged through analyze of the optical image of liquid crystal cell placed onto the surface.更多还原