【Abstract】 A new approach for the determination of methylephedrine hydrochloride(ME),thebaine,codeine phosphate(CP),and acetylcodeine(AC) was established by CE-ECL with ionic liquid(1-butyl-3-methylimidazolium tetrafluoroborate,BMIMBF4). The conditions for the CE separation,ECL detection and the effect of ionic liquid(IL) were systematically investigated. Under the optimal conditions,the four analytes were well separated within 8 min using BMIMBF4 as additive in the electrophoretic buffer. The concentration detection limits of ME,thebaine,CP,and AC were 2.1×10-8,1.4×10-7,6.3×10-8,and 3.6×10-8 mol/L(S/N=3),respectively. The limits of quantitation(LOQ,S/N=10) in real human urine samples were 7.6×10-7 mol/L for ME,3.6 ×10-6 mol/L for thebaine,6.5×10-7 mol/L for CP,and 4.6×10-7 mol/L for AC,respectively. The recoveries of four alkaloids at different levels in human urine samples were between 90.0% and 103.5%. The developed method was successfully applied to the determination of four drug alkaloids in human urine samples.更多还原