【摘要】 利用物理气相沉积法制备了纳米氧化锌(Nano-ZnO)膜,通过扫描电子显微镜(SEM)、紫外-可见分光光度法(UV-Vis)、X射线衍射(XRD)及电化学等方法测定了其物理化学性质.实验结果表明,该Nano-ZnO膜是具有多晶六边形纤维锌矿结构的多孔纳米膜,微粒直径在50～100nm,室温禁带宽度3.37eV.采用浸渍法将超氧化物歧化酶(SOD)直接修饰于Nano-ZnO膜上,制备了SOD修饰电极(SOD/ZnO).通过交流阻抗法(EIS)及循环伏安法(CV)证明了SOD能稳定地吸附在多孔ZnO膜上,并实现了直接电子传递;紫外-可见及红外光谱研究证明吸附在ZnO膜上的蛋白质保持了良好的生物催化活性,并成功地构建了第三代超氧离子(O2-)生物传感器.这种生物传感器有较宽的线性范围(氧化电流:0.24～180×10-6mol/L,还原电流:0.12～250×10-6mol/L)、较低的检测限(氧化电流:2×10-7mol/L,还原电流:1×10-7mol/L)、较快的响应时间(4s)以及较好的稳定性.更多还原

【Abstract】 A nanoporous ZnO film(nano-ZnO) was prepared by physical vapor deposited method and its physical and chemical properties were studied by means of SEM,UV-Vis,XRD and electrochemical methods. The results show that nano-ZnO is a nanoporous film with a polycrystalline hexagonal wurtzite structure. The average diameter of the nanoparticles is about 50—100 nm and its band gap is 3.37 eV. A new superoxide dismutase(SOD) modified nano-ZnO electrode(SOD/ZnO) was prepared by dipping method. SOD was modified stably in the nano-ZnO film confirmed by electrochemical impedance spectroscopy(EIS) and the direct electron transfer could be achieved confirmed by cyclic voltammetry(CV). Spectrum studies indicated that SOD kept its bio-activities. A novel third-generation O-2 biosensor with high selectivity,quick response time(4 s),large linear range(anodic current response:0.24—180×10-6 mol/L; cathodic current response:0.12—250×10-6 mol/L) and good sensitivity(anodic:2×10-7 mol/L; cathodic:1×10-7 mol/L) was constructed and its analytical properties were studied.更多还原